As we have explained in previous posts, magnetic beads can aggregate due to different reasons, bringing problems to the processes they are used for. If you want to learn more about breaking up irreversible magnetic bead aggregates, with techniques such as the sonication method, keep reading!
One of the biggest worries of scientists and technicians in a lab is contamination. Avoiding contamination is best accomplished by using closed mixing vessels (normally closed roller bottles). Closed vessels allow constant mixing and homogenizing without worrying about contacting the suspension. Roller mixing can also be easily scaled up to tens of liters.
This post is about resuspension techniques, such as the sonication method, and how they can solve magnetic bead aggregation. If you are interested in this topic, download our free ebook The basic guide for resuspending magnetic beads:
Contamination can also be a problem if beads are stored for long periods of time. During this time in storage it is important that beads are constantly mixed and homogenized in order to avoid the sedimentation and aggregation that can increase variability and adversely affect in-lot consistency. Because of these potential difficultiess, closed roller bottles and roller mixers are commonly used for long term storage of magnetic beads.
The problem with closed bottles
The main problem of using closed bottles is that buffers need to be changed many times during the production process. During washing steps in biomagnetic separation, for example, the liquid needs to bemanually extracted and replaced with new buffer several times. If mixing and homogenizing processes are performed using roller mixers requiring closed bottles the vessels must be closed tightly after each separation step. If the caps are too loose, or if the seal is compromised in any way, leakage can occur causing a loss of material and variability of the resulting product.
If roller mixers are still the homogenization method of choice, even with the above limitations, there is a way to use them with open bottles. The vessels must be placed in a near vertical position and then tilted for turning. If used in this way, the bottles should not be completely filled in order to avoid spills and contamination. The angle of tilt and the turning speed can be adjusted to guarantee optimal homogenization of the suspension. If the technique is done properly and care is taken to protect the beads and the liquid, one can easily change buffers and maintain the magnetic beads in a homogenized state with little difficulty.
Contamination should be a constant focus and concern when homogenizing magnetic beads or keeping magnetic beads in suspension over long periods of time. Although closed bottles are good protection against contamination during homogenization, constant opening and closing the bottles between wash steps can introduce contamination as well. Using open roller bottles on a tilted roller table affords the user the ability to homogenize without touching the liquid while not losing any of the solution, as long as the bottle angle and speed of rotation is carefully chosen.Loading Likes...